(Same as Computational and Systems Biology M32 and Life Sciences M32.) Lecture, three hours; discussion, one hour. Requisites: Life Sciences 30A, 30B. Not open to students with credit for course 31A, 31B, 32A, or 32B. Designed for life sciences students. Methods and results of single and multivariable calculus essential for quantitative training in biology. Limits, differentiation (single and several variables), optimization, integration and methods of integration, Taylor polynomials and applications to approximation, Taylor and other power series, vector valued functions, gradients, and Lagrange multipliers. P/NP or letter grading.