Lecture, four hours; outside study, eight hours. Requisite: course C205. Builds upon basic concepts of chemical ligation covered in course C205, and focuses on current state-of-art methods and designs for precise bioconjugate formation, especially in context of living cells. Focus on recently developed bioconjugate methods from primary literature, and their applications in bioengineering. Students gain deep understanding of principles of bioconjugation: coupling of biologically active molecules to substrates, devices, or one another, especially for applications in living cells and in vivo. Letter grading.