Lecture, four hours; discussion, one hour. Requisites: course 156, Mathematics 3C or 33A, Physics 1C or 5B. Methods for structure determination of biomacromolecules--proteins and nucleic acids--focusing on X-ray crystallography, cryo-EM imaging, and electron diffraction. Covers mathematical and computational methods used to obtain atomic structural models of biomolecules. Key physical principles include scattering phenomena, interference and diffraction, symmetry, Fourier transforms, and atomic interpretation of molecular structures in three-dimensions. Letter grading.