Lecture, three hours; laboratory, two hours. Requisites: Life Sciences 7A, 7B, 7C, 23L. Over last two decades, there has been explosion of new techniques in light microscopy which has provided us with invaluable tools for biological research. Study of light microscopy techniques currently used in research laboratories. Basics of light microscopy (image formation, magnification, resolution, contrast), widefield and fluorescence microscopy, optical sections (confocal, multi-photon, light-sheet and total internal reflection fluorescence microscope), and super-resolution microscopy. Laboratory sessions include setting up and using simple, rail-based microscope; hands-on time and demonstrations on brightfield/epiflurorescence, confocal, light-sheet and super-resolution microscopes. Letter grading.