Lecture, two hours; discussion, two hours; laboratory, two hours. Introduction to molecular structure of chemical, electrical, and mixed synapses as determined by imaging methods such as electron tomography. Comprehensive review of current principles governing synaptic transmission and balanced account of some of most topical areas of field, such as hemifusion, kiss and run, and fast exocytocis. Laboratory sessions review methods for preparing samples through in-depth analysis of imaging strategies. Computer laboratory sessions allow demonstration of data processing and interpretation. Three round table discussions provide forum for further inspiration as well as tackling any questions or difficulties that may arise from laboratories and lectures. S/U grading.