Lecture, two hours; laboratory, four hours. Requisites: courses 14BL or 20L and 30AL, and 153A or 153AH (may be taken concurrently), with grades of C- or better. Integrated term-long project involving biofuel production in bacteria. Purification of key enzyme for alcohol production from bacteria via affinity chromatography. Assessment of protein amount, purity, and activity of enzyme. Techniques include protein determination by Bradford assay, polyacrylamide gel electrophoresis, immunoblotting, and enzyme activity assays to determine enzyme activity (Km, Vmax, inhibitor studies). P/NP or letter grading.

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Previously taught
24S 23S 22F 22W 21F 21S 21W 20F 18F 18S 17W 16S 16W 15F 15S 15W 14F