Lecture, two hours; laboratory, four hours. Requisites: courses 14BL or 20L and 30AL, and 153A or 153AH (may be taken concurrently), with grades of C- or better. Integrated term-long project involving biofuel production in bacteria. Purification of key enzyme for alcohol production from bacteria via affinity chromatography. Assessment of protein amount, purity, and activity of enzyme. Techniques include protein determination by Bradford assay, polyacrylamide gel electrophoresis, immunoblotting, and enzyme activity assays to determine enzyme activity (Km, Vmax, inhibitor studies). P/NP or letter grading.

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Course

Instructor
Steven Kim
Previously taught
14S 14W 13F 12S 12W 11S 09W 08F 08S 08W 07F 06S 02S 02W 01F

Previous Grades

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